Technological platform

The B Cell Design platform is based on a patented transgenic mouse model (HAMIGA™, GAmmaPrim™ and InEpsTM in collaboration with the B Cell Design model) generated in an academic laboratory (UMR CNRS 7276 - Limoges University - CRIBL – “B cells immune response control and lymphoproliferation”, directed by Pr. Dr. Michel Cogné).
The insertion of a human cassette carrying the gene of the immunoglobulin heavy chain constant region in the endogenous locus leads to the expression of human chimeric antibodies directly by the mouse lymphocytes. . Therefore, these mice are producing antibodies with the following properties:
- only one human isotype per mouse line
- the heavy chain constant region is human
- the variable regions are from the mouse
- the mouse's complete immune repertoire is maintained
There are 3 mouse lines producing human IgA1, IgG1 and IgM/E respectively :
Mouse line | Antibodies produced |
---|---|
HAMIGA™ | Human chimeric IgA1 |
GammaPrim™ | Human chimeric IgG1 |
InEps™ | Human chimeric IgM and IgE by an in vitro induced "class switching" |